1. Trihydroxymethyl aminomethane ee jihada synthesis warshadaha
Sababtoo ah qaab-dhismeedkeeda fudud, atamka dhexe ee kaarboonku waa qaab-dhismeedka tetrahedral, oo ka kooban saddex hydroxymethyl iyo koox amino ah, kuwaas oo fulin kara noocyo kala duwan oo beddelaad ah iyo falcelinta REDOX, waa nooc ka mid ah qalabka kiimikada ee tayada sare leh. Mid ka mid ah hababka isku dhafka ah ee TAPS kaydinta ayaa la sameeyay iyadoo la adeegsanayo Tris iyo 1, 3-propanolone ee khamriga. Caadiyan, alaabta ceyriinka ah ee TRIS ee loo isticmaalo isku-dhafka warshadaha uma baahna nadiifin sare oo reagent ah. Culaysku waa tiro badan, markaa way ka kharash badan tahay in la helo saaraha alaabta ceeriin leh oo leh awood weyn.
2. Trimethylol amino methane ee jihada ogaanshaha biochemical
Tris waxaa sida caadiga ah loo isticmaalaa sidii kayd bayooloji ah si loo ilaaliyo pH ee falcelinta biochemical. Sababtoo ah alkalinity daciif ah, Tris waxaa badanaa loo isticmaalaa marka lagu daro acids. Waxaa inta badan loo isticmaalaa nucleic acid, borotiinka kala diridda bakhtiyaa, borotiinka imprinting buffer, DNA imprinting tijaabo kaydiyaha, DNA agarose gel electrophoresis buffer, iwm. In nidaamka falcelinta sababtoo ah ku lug leh enzymes, borotiinada iyo unugyada difaaca ee kala duwan, sidaas darteed shuruudaha kaydinta waa xad sare, sidaas darteed iibsigu wuxuu u baahan yahay inuu doorto soo saaraha sanado badan oo cilmi baaris iyo horumarin iyo khibrad wax soo saar leh, nadiif sare, wasakh yar si loo hubiyo saxnaanta tijaabooyinka biochemical. Saamaynta heerkulka Tris baffer, fududahay in la nuugo kaarboon dioxide hawada, qaabaynta kaydiyaha waa in si adag loo daboolaa.
3. Trimethylol aminomethane ee cilmi-baarista dawooyinka iyo horumarinta
Saddex hydroxymethyl aminomethane alias kale, kuwaas oo ah trometamol, sida aan ku jirin sodium amino buffer base, in dheecaannada jidhka ka falcelin H2CO3, yareeyaan H2CO3, abuuraan HCO32 - mar kale, nuugi karo ions hydrogen iyo sax acidemia, saamayntiisa waa ka sii xoog badan. , waxayna dhex mari kartaa xuubka unugyada, oo inta badan loo isticmaalo dheef-shiid kiimikaad ba'an iyo aashitada neefsiga; Sida daroogo alkaline ah, loo isticmaalo sixitaanka acidosis iyada oo aan keenin korodhka sii-haysashada carbon dioxide. Sababtoo ah waxaa loo isticmaalaa dawooyinka, wax soo saarka oo dhan TRIS isha alaabta ceeriin, habka wax soo saarka, daahirsanaanta alaabta, content ion wasakh ah waa shuruudo adag, soo iibinta alaabta noocan oo kale ah waa in ay ahaataa mid aad u adag oo taxadar leh, waa in ay gudbaan imtixaan adag.
4. Trimethylaminomethane waxa loo isticmaalaa dareeraha kaydinta fayraska
Xalka ilaalinta fayraska, Tris waxa uu marka hore ilaalin karaa qiimaha pH ee muunadda, isaga oo u dhaqmaya sidii kayd bayooloji ah, iyo kala duwanaanta pH-ka ee Tris waa: 7.0 ilaa 9.0, awoodda lagu ilaalinayo soo saarista muunadda dildilaaca ka dib marka la sii daayo xasiloonida aashitada nucleic, si looga fogaado hoos u dhaca nucleic acid, iyo hagaajinta diiradda iyo daahirsanaanta nucleic acid, dammaanad sax ahaanshaha hawlgallada soo socda sida falanqaynta baaritaanka aashitada nucleic, sababta oo ah muddada cudurka faafa ee gaarka ah, shuruudaha ogaanshaha nucleic acid, Tris tirada iibsigu way ka sii weynaan doontaa, markaa soo jeedi iibsashada alaabooyinkan oo kale waa inay marka hore iibsadaan qadar yar oo badeecada ka hor inta aanad iibsan tijaabo weyn oo la xaqiijiyay in aan wax dhibaato ah lahayn, sidoo kale waa in uu leeyahay farsamo iib ka dib si uu u bixiyo taageero farsamo oo xirfad leh.
Waqtiga boostada: Febraayo-24-2021