N,N-bis(2-chloroethyl)carbamate tert-butyl ester waxaa loo isticmaali karaa dhexdhexaad ahaan kiimikada dawooyinka iyo isku-darka dabiiciga ah. Isbeddellada isku-darka dabiiciga ah, kooxda Boc ee qaab-dhismeedka si fudud ayaa looga saari karaa si loo helo koox karboksil firfircoon.

Habka isku-dhafka ah wuxuu ku lug leeyahay in si degdeg ah loogu walaaqo joojinta bis-(2-chloroethyl)amine hydrochloride (5 g, 0.028 mol) ee dichloromethane (42 mL) iyadoo la isticmaalayo10% sodium hydroxide xal biyood ah (28 mL) heerkulka qubeyska barafka. Xal di-tert-butyl dicarbonate ah (6.11 g, 0.028 mol) oo ku jira dichloromethane (28 mChemicalbook L) ayaa markaa lagu daraa. Ka dib marka heerkulka qolka lagu walaaqo 18.5 saacadood, dichloromethane (30 mL) ayaa si tartiib ah loogu daraa isku darka falcelinta. Labada marxaladoodba waa la kala saaraa. Marxaladda biyaha waxaa si dheeraad ah looga soo saaraa dichloromethane (30 mL). Lakabyada dabiiciga ah ee isku dhafan ayaa lagu qalajiyaa magnesium sulfate aan biyo lahayn, la sifeeyaa, oo la ururiyaa si loo helo N,N-bis(2-chloroethyl)carbamate tert-butyl.